Isolation and characterization of new porphyrin metabolites in human porphyria cutanea tarda and in rats treated with hexachlorobenzene by HPTLC, HPLC and liquid secondary ion mass spectrometry

✍ Scribed by Jinli Luo; Chang Kee Lim

• Publisher: John Wiley and Sons
• Year: 1995
• Tongue: English
• Weight: 1002 KB
• Volume: 9
• Category: Article
• ISSN: 0269-3879
• DOI: 10.1002/bmc.1130090302

✦ Synopsis

Porphyrin metabolisms in human porphyria cutanea tarda (PCT) and in rats treated with hexachlorobenzene (HCB) have been studied in detail by high performance thin layer chromatography (HPTLC), high performance liquid chromatography (HPLC) and liquid secondary ion mass spectrometry (LSTMS). The analyses of porphyrin metabolites in the urine, faeces and liver biopsies of patients with PCT have shown that apart from uroporphyrin I and I11 and their expected decarboxylation intermediates and products, a complex mixture of many other porphyrins are present. The new porphyrins identified are: meso-hydroxyuroporphyrin 111, p hydroxypropionic acid uroporphyrin 111, hydroxyacetic acid uroporphyrin 111, peroxyacetic acid uroporphyrin 111, 6-hydroxypropionic acid heptacarboxylic acid porphyrin 111, hydroxyacetic acid heptacarboxylic porphyrin 111 and peroxyacetic acid pentacarboxylic porphyrin 111.