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Isolation and characterization of mutantPseudomonas aeruginosastrains unable to assimilate nitrate

โœ Scribed by Randall M. Jeter; John L. Ingraham

Publisher
Springer
Year
1984
Tongue
English
Weight
700 KB
Volume
138
Category
Article
ISSN
0302-8933

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โœฆ Synopsis

Single-site mutants of Pseudomonas aeruginosa that lack the ability aerobically to assimilate nitrate and nitrite as sole sources of nitrogen have been isolated. Twenty-one of these have been subdivided into four groups by transductional analysis. Mutants in only one group, designated nis, lost assimilatory nitrite reductase activity. Mutants in the other three transductional groups, designated ntmA, ntmB, ntmC, display a pleiotropic phenotype: utilization of a number of nitrogen-containing compounds including nitrite as sole nitrogen sources is impaired. Assimilatory nitrite reductase was shown to be the major route by which hydroxylamine is reduced in aerobically-grown cells.

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Isolation and analysis of mutants ofPseudomonas aeruginosaunable to assimilate nitrate
โœ Stacey R. Sias; John L. Ingraham ๐Ÿ“‚ Article ๐Ÿ“… 1979 ๐Ÿ› Springer ๐ŸŒ English โš– 712 KB

Pseudomonas aeruginosa can reduce nitrate to nitrite and eventually to nitrogen gas by the denitrification pathway, thereby providing the organism with a mode of respiration and ATP generation in the absence of oxygen. P. aeruginosa can also reduce nitrate to nitrite through an assimilatory pathway