Isolation and characterization of Locusta migratoria accessory gland myotropin I (Lom-AG-MT-I) from the brain of the Colorado potato beetle, Leptinotarsa decemlineata
✍ Scribed by Kurt Spittaels; Anne Vankeerberghen; Liliane Schoofs; Paul Proost; Jozef Van Damme; Arnold De Loof
- Publisher
- John Wiley and Sons
- Year
- 1996
- Tongue
- English
- Weight
- 393 KB
- Volume
- 31
- Category
- Article
- ISSN
- 0739-4462
No coin nor oath required. For personal study only.
✦ Synopsis
A novel myotropic Colorado potato beetle peptide, active in the Locusta oviduct motility assay, was isolated from a methanolic extract of 9,000 brain complexes of adult Leptinofarsa decemlineata by means of HPLC. Its sequence is Gly-Phe-Lys-Asn-Val-Ala-Leu-Ser-Thr-Ala-Arg-Gly-Phe-NH~. This peptide is identical to Lom-AG-MT-I, a myotropin previously isolated from the male accessory glands of Locusta migratoria, using the L. rnigratoria oviduct motility bioassay as a monitoring system. It strongly stimulated the frequency, amplitude, and tonus of the myogenic oviduct contractions, even at low concentrations.