Isolation and Characterization of Insulin-like Growth Factor-I from Rainbow Trout, Oncorhynchus mykiss
β Scribed by Shunsuke Moriyama; Walton W. Dickhoff; Erika M. Plisetskaya
- Book ID
- 112245961
- Publisher
- Elsevier Science
- Year
- 1995
- Tongue
- English
- Weight
- 372 KB
- Volume
- 99
- Category
- Article
- ISSN
- 0016-6480
No coin nor oath required. For personal study only.
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## Abstract A recombinant human IGFβI (rhIGFβI) was applied to primary cultures of rainbow trout pituitary cells. In wells containing 3 Γ 10^4^ and 6 Γ 10^4^ cells/well, rhIGFβI inhibited basal GH release both in short (6 h) and long (12 and 24 h) exposures. The decline in GH release was doseβdepen
## Abstract Recombinant human IGFβI (hIGFβI) binding to rainbow trout serum fractions was studied in vitro. Binding protein preparations, from human and trout serum (hBP and tBP), which were obtained using size exclusion chromatography (AcA 54) under acid conditions, were used to characterize IGF b