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Isolation and Characterization of Insulin-like Growth Factor-I from Rainbow Trout, Oncorhynchus mykiss

✍ Scribed by Shunsuke Moriyama; Walton W. Dickhoff; Erika M. Plisetskaya


Book ID
112245961
Publisher
Elsevier Science
Year
1995
Tongue
English
Weight
372 KB
Volume
99
Category
Article
ISSN
0016-6480

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## Abstract A recombinant human IGF‐I (rhIGF‐I) was applied to primary cultures of rainbow trout pituitary cells. In wells containing 3 Γ— 10^4^ and 6 Γ— 10^4^ cells/well, rhIGF‐I inhibited basal GH release both in short (6 h) and long (12 and 24 h) exposures. The decline in GH release was dose‐depen

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## Abstract Recombinant human IGF‐I (hIGF‐I) binding to rainbow trout serum fractions was studied in vitro. Binding protein preparations, from human and trout serum (hBP and tBP), which were obtained using size exclusion chromatography (AcA 54) under acid conditions, were used to characterize IGF b