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Isolation and Characterization of Human Milk Bile Salt-Activated Lipase C-Tail Fragment

✍ Scribed by Wang, Chi-Sun; Dashti, Azar; Jackson, Ken W.; Yeh, Jiunn-Chern; Cummings, Richard D.; Tang, Jordan


Book ID
126110267
Publisher
American Chemical Society
Year
1995
Tongue
English
Weight
776 KB
Volume
34
Category
Article
ISSN
0006-2960

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A modified procedure for a large-scale purification of human milk bile salt-activated lipase (BAL) has been devised. An initial step used cholate-Sepharose affinity chromatography for the partial purification of the enzyme followed by the removal of cholate with a Bio-Rex 5 anionexchange resin. The