Purification of human milk bile salt-act
β
Chi-Sun Wang; Kerri Johnson
π
Article
π
1983
π
Elsevier Science
π
English
β 455 KB
A modified procedure for a large-scale purification of human milk bile salt-activated lipase (BAL) has been devised. An initial step used cholate-Sepharose affinity chromatography for the partial purification of the enzyme followed by the removal of cholate with a Bio-Rex 5 anionexchange resin. The