Isolation and characterization of glycoprotein lectins from the bark of three species of elder,Sambucus ebulus, S. nigraandS. racemosa
✍ Scribed by M. Nsimba-Lubaki; W. J. Peumans; A. K. Allen
- Book ID
- 104760915
- Publisher
- Springer-Verlag
- Year
- 1986
- Tongue
- English
- Weight
- 797 KB
- Volume
- 168
- Category
- Article
- ISSN
- 0032-0935
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✦ Synopsis
Lectins have been isolated from the bark of three members of the family Caprifoliaceae, Sambucus nigra (elder), S. racemosa (red-berried elder) and S. ebulus (dwarf elder), by affinity chromatography on fetuin-agarose, ion-exchange and gel-filtration chromatography. They are all glycoproteins of Mr 140000 made up of at least four subunits. The lectins have similar but not identical amino-acid compositions and the carbohydrate content varies between 12% and 19% (w/w), the main sugars being (N-acetyl)glucosamine, mannose, fucose and xylose. Inhibition studies of hemagglutination with various mono-and oligosaccharides have shown that N-acetylgalactosamine and galactose together with galactose-containing oligosaccharides are the most effective inhibitors. There are some differences in specificity, in particular S. ebulus agglutinin is inhibited to the same degree by galactosamine, N-acetylgalactosamine and by galactose.