Isolation and characterization of copper-binding sites of human ceruloplasmin
โ Scribed by K. S. Raju
- Publisher
- Springer
- Year
- 1983
- Tongue
- English
- Weight
- 586 KB
- Volume
- 56
- Category
- Article
- ISSN
- 0300-8177
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Human ceruloplasmin, which is usually cleaved by limited proteolysis into three major fragments during preparation (M, = 18,650,5O,OOO, and 70,OOO) was isolated in good yield as an undegraded single-chain protein (M, = 135,000). The cryosupernatant from fresh frozen plasma (100 liters) was fractiona
The three-dimensional molecular structure of human serum ceruloplasmin has been reinvestigated using X-ray synchrotron data collected at 100 K from a crystal frozen to liquid-nitrogen temperature. The resulting model, with an increase in resolution from 3.1 to 2.8 A, gives an overall improvement of