Isolation and Characterization of Chemotaxis Mutants ofChlamydomonas reinhardtii
β Scribed by Elena V. Ermilova; Elena M. Chekunova; Zhanna M. Zalutskaya; Konstantin R. Krupnov; Boris V. Gromov
- Book ID
- 105997804
- Publisher
- Springer
- Year
- 1996
- Tongue
- English
- Weight
- 54 KB
- Volume
- 32
- Category
- Article
- ISSN
- 0343-8651
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A rapid procedure has been developed for the isolation of the photosystem two reaction centre complex (PS II RC) from a double mutant of Chlamydomonas reinhardtii, F54-14, which lacks the Photosystem one complex and the chloroplast ATPase. Thylakoid membranes are solubilised with 1.5% (w/v) Triton X
The synthesis of polypeptides related to the CPI chlorophyll-protein complex of photosystem I has been studied by pulse-labeling experiments in twenty chloroplast mutants of Chlamydomonas reinhardtii. Three mutations of the same locus (Girard-Bascou 1987) result in the absence of these CPI-related p