✦ LIBER ✦

Isolation and characterization of alginate-derived oligosaccharides with root growth-promoting activities

✍ Scribed by Midori Natsume; Yoshihiro Kamo; Masao Hirayama; Takashi Adachi

Publisher: Elsevier Science
Year: 1994
Tongue: English
Weight: 716 KB
Volume: 258
Category: Article
ISSN: 0008-6215
DOI: 10.1016/0008-6215(94)84085-7

No coin nor oath required. For personal study only.

✦ Synopsis

Lytic digestion of poly(mannuronate), poly(guluronate1, and alginate with an alginate lyase from Alteromonas maclwdii was used to prepare mixtures of unsaturated oligosaccharides. Four oligosaccharides isolated from the alginate lyase-lysate by anion-exchange chromatography on Q-Sepharose were found to be the major components of the root growth-promoting lysate. The oligosaccharides were analyzed by NMR and SIMS and identified as di-and tri-saccaharides having O-(4-deoxy-L-erythro-hex-4-enopyranosyluonic acid)-1 + at the nonreducing terminus. The trisaccharides from the lysate were found to have root growth-promoting activity in a barley bioassay.

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