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Isolation and characterization of abscisic acid-deficient Arabidopsis mutants at two new loci

✍ Scribed by Karen M. Léon-Kloosterziel; Marta Alvarez Gil; Gerda J. Ruijs; Steven E. Jacobsen; Neil E. Olszewski; Steven H. Schwartz; Jan A.D. Zeevaart; Maarten Koornneef

Publisher
John Wiley and Sons
Year
1996
Tongue
English
Weight
937 KB
Volume
10
Category
Article
ISSN
0960-7412

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✦ Synopsis

Novel Arabidopsis mutants with lowered levels of endogenous abscisic acid (ABA) were isolated. These were selected in a screen for germination in the presence of the gibberellin biosynthesis inhibitor paclobutrazol. Another mutant was isolated in a screen for NaCl tolerance. The ABA‐deficiency was caused by two monogenic, recessive mutations, aba2 and aba3, that were both located on chromosome 1. The mutants showed a phenotype that is known to be characteristic for ABA‐deficiency: a reduced seed dormancy and excessive water loss, leading to a wilty phenotype. Double mutant analysis, combining different aba mutations, indicated the leaky nature of the mutations.

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