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Isolation and characterization of a vitellogenesis-inhibiting factor from sinus glands of the lobster,Homarus americanus

✍ Scribed by Soyez, D. ;Van Deijnen, J. E. ;Martin, M.

Publisher: John Wiley and Sons
Year: 1987
Tongue: English
Weight: 525 KB
Volume: 244
Category: Article
ISSN: 0022-104X
DOI: 10.1002/jez.1402440314

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✦ Synopsis

A vitellogenesis-inhibiting 0 factor has been characterized from extracts of lobster sinus glands (SG) using an in vivo bioassay developed in the shrimp, Palaemonetes varians. After a two-step purification by reversedphase high-performance liquid chromatography mP-HPLC), the bioactivity is recovered in one symmetrical peak. Sodium dodecyl sulfate-urea-polyacryylamide gel electrophoresis (SDS-urea-PAGE) of the bioactive fractions reveals, after silver staining, the presence of only one band corresponding to a 7-8 kD peptide.

The crustacean neuroendocrine complex, Xorgan-sinus gland (SG), is known to be the

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