Isolation and characterization of a virulent phage forRhodobacter sphaeroides

✍ Scribed by Michael Duchrow; Stefan Heitefuss; Jutta Kalkus; Michael Hoppert; Friedrich Giffhorn

• Publisher: Springer
• Year: 1988
• Tongue: English
• Weight: 551 KB
• Volume: 149
• Category: Article
• ISSN: 0302-8933
• DOI: 10.1007/bf00425591

✦ Synopsis

A new virulent bacteriophage, termed ~RsV, was isolated from a local sewage plant on the facultative phototrophic bacterium Rhodobacter sphaeroides DSM 159 as the host organism. Electron microscopic studies revealed that in general morphology phage oRsV resembles the T-even Escherichia coli phages. The host range of phage oRsV was restricted to strains of R. sphaeroides. E. coli strains B and K 12 were not infected. The phage genome was characterized on the basis of thermal denaturation profiles and restriction analyses indicating that it consists of about 160 kb of double-stranded DNA lacking cohesive ends. The G + C content was determined to be 46.8 tool%.