Isolation and characterization of a pleiotropic glucose repression resistant mutant of Saccharomyces cerevisiae

✍ Scribed by Bailey, Richard B. ;Woodword, Anne

• Publisher: Springer
• Year: 1984
• Tongue: English
• Weight: 694 KB
• Volume: 193
• Category: Article
• ISSN: 0026-8925
• DOI: 10.1007/bf00382091

✦ Synopsis

A new mutation has been described which confers resistance to catabolite repression in Saccharomyces cerecisiae. The mutant allele, termed grr-I for glucose repression-resistant, is characterized by insensitivity to glucose repression for the cytoplasmic enzymes invertase, maltase, and galactokinase, as well as the mitochondrial enzyme cytochrome c oxidase. Hexokinase levels in grr-I mutants are approximately 3-tbld higher than the corresponding activity of the parental strain. Although the grr-I allele is expressed phenotypically similarly to the hex-I (hxk-2) and hex-2 mutations described by Entian et al. (1977) and Zimmermann and Scheel (1977) respectively, we have shown genetically and physiologically that grr-I represents a new class of mutation.