Isolation and characterization of a novel TP53-inducible gene, TP53TG5, which suppresses growth and shows cell cycle-dependent transition of expression

✍ Scribed by Shigeyuki Isaka; Yoshiki Takei; Takashi Tokino; Kumiko Koyama; Yasuo Miyoshi; Mikio Suzuki; Eiichi Takahashi; Chihiro Azuma; Yuji Murata; Yusuke Nakamura

• Publisher: John Wiley and Sons
• Year: 2000
• Tongue: English
• Weight: 227 KB
• Volume: 27
• Category: Article
• ISSN: 1045-2257
• DOI: 10.1002/(sici)1098-2264(200004)27:4<345::aid-gcc2>3.0.co;2-3

✦ Synopsis

Through a strategy of direct cloning of TP53-binding DNA sequences from human genome DNA, we have identified a novel TP53-target gene, termed TP53TG5 (TP53-target gene 5). This gene, localized to chromosome band 20q13.1 by fluorescence in situ hybridization, encodes a 290-amino-acid peptide with no significant homology with any known proteins in the public database. A colony-formation assay using human glioblastoma cell line T98G, which lacks wild-type TP53 and expresses no endogenous TP53TG5, revealed a growth-suppressive effect of the TP53TG5 gene product. Furthermore, immunohistochemical studies, following transfection of T98G with plasmid designed to express green fluorescent protein-fused TP53TG5, revealed cell cycle-dependent intracellular localization of this protein. Our results suggest that functional studies of TP53TG5 may provide new insights into the complex physiological activities of TP53.