Isolation and Characterization of a GDSL Esterase from the Metagenome of a Marine Sponge-associated Bacteria
โ Scribed by Yoshiko Okamura; Tomonori Kimura; Hiroko Yokouchi; Macarena Meneses-Osorio; Masaya Katoh; Tadashi Matsunaga; Haruko Takeyama
- Publisher
- Springer
- Year
- 2009
- Tongue
- English
- Weight
- 229 KB
- Volume
- 12
- Category
- Article
- ISSN
- 1053-6426
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๐ SIMILAR VOLUMES
Fractionation of the EtOH extract of the marine sponge Discodermia calyx led to the isolation of dephosphonocalyculin A (2). The gross structure 2 was deduced on the basis of spactral data. The structure including the absolute stereochemistry has been confirmed by chemical transformation of 2
## Abstract A 2โhaloacid dehalogenaseโproducing bacterium, designated DEH99, was isolated from the marine sponge __Hymeniacidon perlevis__ using a modified enrichment medium and a pH indicator method. DEH99 could degrade only half of the racemic mixture 2โchloropropionic acid (2โCPA) in the medium.