Isolation and characterization of a female-specific DNA marker in the giant freshwater prawn Macrobrachium rosenbergii
โ Scribed by Ventura, T; Aflalo, E D; Weil, S; Kashkush, K; Sagi, A
- Book ID
- 109862125
- Publisher
- Nature Publishing Group
- Year
- 2011
- Tongue
- English
- Weight
- 438 KB
- Volume
- 107
- Category
- Article
- ISSN
- 0018-067X
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## Abstract A cDNA encoding vitellogenin (Vg) in the giant freshwater prawn, __Macrobrachium rosenbergii,__ was cloned based on the cDNA sequence of vitellin (Vn) fragments AโN and Bโ42 determined previously, and its amino acid sequence deduced. The open reading frame (ORF) encoded 2,537 amino acid
Four major yolk proteins, designated as vitellins (Vns) Macr-VnA, B, C, and D, were extracted from mature ovaries of Macrobrachium rosenbergii. These were purified to homogeneity by reversed-phase high performance liquid chromatography (HPLC) employing a unique separation system based on the hydroph