A method of isolation of rat liver cells and mitochondria suggesting tissue disaggregation by vibration has been proposed. Mitochondria obtained using vibration have better parameters of oxidative phosphorylation as compared to homogenization. Hepatocytes isolated by vibration had viability about 90
Isolation and characteristics of hepatocytes
โ Scribed by G. Van De Werve
- Publisher
- Elsevier Science
- Year
- 1980
- Tongue
- English
- Weight
- 296 KB
- Volume
- 18
- Category
- Article
- ISSN
- 0300-483X
No coin nor oath required. For personal study only.
๐ SIMILAR VOLUMES
Oxygen uptake rate (OUR) of hepatocytes is an important parameter for the design of bioartificial liver assist (BAL) devices. Porcine hepatocytes were cultured in a specially constructed measurement chamber with an incorporated mixing system and a Clark polarographic oxygen electrode. Signal noise a
Abbreviations: IPRL, isolated perfused rat liver preparation; P 0 , zero-flow intercept of the regression line calculated from pressure-flow data relating to the flow rates above 20 mL/min; G max , maximum portal vascular conductance or the reciprocal gradient of the regression line above; H A , hep