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Isolation and characterisation of a rhamnogalacturonan II from red wine

✍ Scribed by Thierry Doco; Jean-Marc Brillouet

Publisher: Elsevier Science
Year: 1993
Tongue: English
Weight: 720 KB
Volume: 243
Category: Article
ISSN: 0008-6215
DOI: 10.1016/0008-6215(93)87037-s

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✦ Synopsis

A complex polysaccharide originating from grape was purified from a red wine by size-exclusion and ion-exchange chromatography. This polysaccharide contained 2-O-methylfucose, rhamnose, fucose, 2-O-methylxylose, arabinose, apiose, galactose, galacturonic and glucuronic acids, and thiobarbituric acid assay-positive material, presumably 3-deoxy-o-manno-2-octulosonic acid (Kdo) and 3-deoxy+!yXO-2-heptulosaric acid (Dha). Methylation structural analysis showed that this polysaccharide is a typical rhamnogalacturonan II (RG-II), a structurally complex pectic polysaccharide that has been isolated previously from the primary cell walls of sycamore. Red wine RG-II presented discrete differences from previously described RG-II's, a greater richness in uranic acids and some additional glycosyl linkages, which might be related to the pattern of degradation of grape-wall pectic substances by endogenous pectinolytic enzymes after pressing the berries and subsequent fermentation to wine.

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