Isolation and biochemical diagnosis of cell lines of groundnut ( Arachis hypogaea L) selected on glyphosate†
✍ Scribed by Jain, Mukesh; Bhatnagar, Raj K; Sarin, Neera B
- Publisher
- John Wiley and Sons
- Year
- 1999
- Tongue
- English
- Weight
- 142 KB
- Volume
- 55
- Category
- Article
- ISSN
- 1526-498X
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✦ Synopsis
Three cell lines of groundnut (Arachis hypogaea L), an important oilseed legume, were selected on glyphosate using in-vitro culture techniques. The cell lines isolated through single as well as stepwise selection procedures showed c 20-fold increase in glyphosate tolerance as compared to the unselected control cell line. Studies on the biochemical mechanism of glyphosate tolerance in these cell lines showed a signi®cant increase in the total extractable activity of the target enzyme, 5-enolpyruvyl shikimate-3-phosphate (EPSP) synthase (EC 2.5.1.19), which was further con®rmed with immunological data. The over-expressed EPSP synthase activity was, however, subject to inhibition by glyphosate in vitro. Two other key regulated enzymes of the shikimic acid pathway, 3-deoxy-D-arabino heptulosonate 7-phosphate (DAHP) synthase (EC 4.1.2.15) and chorismate mutase (CM) (EC 5.4.99.5) did not show any change in speci®c activity in the selected cell lines. The enhanced activity of EPSP synthase in the tolerant cell lines was found to be stably inherited in the absence of selection pressure.