Isolation and assay of red-cell inositol polyphosphates
โ Scribed by Grant R. Bartlett
- Publisher
- Elsevier Science
- Year
- 1982
- Tongue
- English
- Weight
- 476 KB
- Volume
- 124
- Category
- Article
- ISSN
- 0003-2697
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๐ SIMILAR VOLUMES
SiJMMARY ## Gel chromatography of inositol polyphosphates has shown that a substantial anion exclusion effect exists which is diminished, but not necessarily eliminated, by concentrations of eluant electrolyte up to 2 M. Under the appropriate conditions of ionic'strength and pH; gel chromatograph
Fluid charged lipid vesicles loaded with an inositol hexaphosphate solution were used to transport this allosteric effector into human intact red blood cells. Rate and extent of uptake of the vesicles and of the effector by the red blood cells were measured as changes in the O2 half-saturation press