Isolated hapten-binding receptors of sensitized lymphocytes. IV. Expression of immimoglobulin variable regions in (4-hydroxy-3-nitrophenyl) acetyl (NP)-specific receptors isolated from murine B and T lymphocytes
✍ Scribed by Matthias Cramer; Ulrich Krawinkel; Inga Melchers; Thereza Imanishi-Kari; Klaus Rajewsky; Yinon Ben-Neriah; David Givol
- Publisher
- John Wiley and Sons
- Year
- 1979
- Tongue
- English
- Weight
- 782 KB
- Volume
- 9
- Category
- Article
- ISSN
- 0014-2980
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✦ Synopsis
Abstract
Primary anti‐NP [(4‐hydroxy‐3‐nitrophenyl)acetyl] antibodies and NP‐specific receptors isolated from T cells of mice carrying the Ig‐1^b^ allotype express the NP^b^ idio‐type and a fine specificity marker (heteroclicity) which are both controlled by V genes in the heavy chain linkage group. While in antibodies both markers are invariably expressed in association with Ig light chains of the Λ type, antigen‐binding T cell‐derived receptors appear not to follow this rule in that they do not significantly bind to anti‐Λ antisera.
Mice of the SJL strain also carry the Ig‐l^b^ allotype but express a defect in Λ chain synthesis, and their primary anti‐NP response is thus almost completely devoid of Λ‐bearing heteroclitic molecules of the NP^b^ idiotype. In contrast, both NP^b^ idiotype and heteroclicity are expressed in the majority of T cell‐derived receptors. This result permits a positive discrimination between T cell‐derived receptors and humoral antibodies in the SJL strain and shows that V gene expression follows different rules in the T and the B cell compartment.
The expression of Ig V regions in isolated T cell‐derived receptors was also analyzed with antisera binding to presumed “framework” determinants of the V~H~ and the V~Λ~domains of Ig. A reaction of anti‐V~H~ antiserum with isolated T cell receptors could clearly be demonstrated. The anti‐V~Λ~ antiserum may bind to a minor fraction of the NP‐specific molecules in our T cell receptor preparations.