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Isolated cortical granules: A model system for studying membrane fusion and calcium-mediated exocytosis

✍ Scribed by Vacquier, Victor D.


Publisher
Wiley (John Wiley & Sons)
Year
1976
Tongue
English
Weight
648 KB
Volume
5
Category
Article
ISSN
0091-7419

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✦ Synopsis


Abstract

Cortical granules are secretory vesicles bound to the inner surface of the plasma membrane of sea urchin eggs. Intact granules can be isolated by shearing away the cytoplasm of eggs which have been bonded to a protamine‐coated surface. When Ca^2+^ is added to preparations of isolated granules the granules fuse with each other and release their contents. It is believed that isolated cortical granules may be an excellent model system for the biochemical study of exocytosis.