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Isoforms of an N-acetyl-β-d-glucosaminidase from the Antarctic krill, Euphausia superba: purification and antibody production

✍ Scribed by Gerrit Peters; Reinhard Saborowski; Rolf Mentlein; Friedrich Buchholz


Book ID
117485785
Publisher
Elsevier Science
Year
1998
Tongue
English
Weight
219 KB
Volume
120
Category
Article
ISSN
1096-4959

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The enzyme N-acetyl-0-D-glucosaminidase was purified from the cortical granules of Xenopus laevis eggs using affinity chromatography, gel filtration, and density gradient centrifugation. The enzyme had a molecular weight of 37,000-40,000 as determined by polyacrylamide gel electrophoresis and densit