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Isoenzyme pattern and de novo synthesis of phosphodiesterase during differentiation (spherulation) in Physarum polycephalum

✍ Scribed by H�ttermann, A.

Publisher: Springer-Verlag
Year: 1972
Weight: 534 KB
Volume: 83
Category: Article
ISSN: 0003-9276
DOI: 10.1007/bf00425022

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✦ Synopsis

Under conditions of CsCl-equilibrium sedimentation, phosphodiesterase in extracts made from growing Physarum microplasmodia forms two bands with buoyant densities of 1.3572 g/ml (Phosphodiesterase I) and 1.2937 g/ml (Phosphodiesterase II). In spherulating cultures induced by starvation, only phosphodiesterase I is present and true de novo synthesis of this enzyme during this differentiation was demonstrated by density labeling with deuterated amino acids. The synthesis is inhibited by cyeloheximide, whereas only the total activity but not the density of the enzyme was influenced by actinomycin-C.

In spherulating cultures induced by mannitol both isoenzymes are present as in the growing cultures.

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