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Isoelectric focusing of proteins using a pH gradient created by a concentration gradient of nonelectrolytes in solution

✍ Scribed by G.V. Troitsky; V.P. Zav'yalov; I.F. Kirjukhin; V.M. Abramov; G.Ju. Agitsky


Book ID
113124830
Publisher
Elsevier Science
Year
1975
Weight
413 KB
Volume
400
Category
Article
ISSN
0005-2795

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Isoelectric focusing of proteins in a mi
✍ Jiaqi Wu; Janusz Pawliszyn πŸ“‚ Article πŸ“… 1992 πŸ› John Wiley and Sons 🌐 English βš– 296 KB

## Abstract Proteins were separated by capillary isoelectric focusing (CIEF) performed in a 5‐μm i.d., 12‐cm long uncoated capillary, and detected by an on‐column concentration gradient detector based on Schlieren optics. The narrow capillary allows the application of a 15‐kV separation voltage. Th