✦ LIBER ✦

Isoelectric focusing and non-isoelectric precipitation of ferritin in immobilized pH gradients: An improved protocol overcoming protein-matrix interactions

✍ Scribed by Prof. Pier Giorgio Righetti; Cecilia Gelfi; Maria Luisa Bossi; Egisto Boschetti

Publisher: John Wiley and Sons
Year: 1987
Tongue: English
Weight: 933 KB
Volume: 8
Category: Article
ISSN: 0173-0835
DOI: 10.1002/elps.1150080112

No coin nor oath required. For personal study only.

📜 SIMILAR VOLUMES

An optimized procedure for detection of

An optimized procedure for detection of proteins on carrier ampholyte isoelectric focusing and immobilized pH gradient gels with imidazole and zinc salts: Its application to the identification of isoelectric focusing separated isoforms by in-gel proteolysis and mass spectrometry analysis

✍ Lila Castellanos-Serra; Antonio Vallin; Wilfredo Proenza; Jean Pierre Le Caer; J 📂 Article 📅 2001 🏛 John Wiley and Sons 🌐 English ⚖ 131 KB 👁 2 views

Two-dimensional electrophoretic separati

Two-dimensional electrophoretic separation of yeast proteins using a non-linear wide range (pH 3–10) immobilized pH gradient in the first dimension; reproducibility and evidence for isoelectric focusing of alkaline (pI >7) proteins

✍ Norbeck, Joakim; Blomberg, Anders 📂 Article 📅 1997 🏛 John Wiley and Sons 🌐 English ⚖ 346 KB 👁 2 views

The proteome of the yeast Saccharomyces cerevisiae was analysed by two-dimensional (2D) polyacrylamide gel electrophoresis utilizing a non-linear immobilized pH gradient (3-10) in the first-dimensional separation. Cells were labelled by [ 35 S]methionine incorporation in the respiro-fermentative pha