Isocratic reversed-phase liquid chromatography for assay of 5'-nucleotidase with phosphoric acid for reaction quenching
โ Scribed by Yu, Li Wen.; Fager, Roger S.
- Book ID
- 126144528
- Publisher
- American Chemical Society
- Year
- 1982
- Tongue
- English
- Weight
- 384 KB
- Volume
- 54
- Category
- Article
- ISSN
- 0003-2700
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๐ SIMILAR VOLUMES
A high-performance liquid chromatographic method for the simultaneous determination of the amino acids methionine, valine, tryptophan, phenylalanine, isoleucine, and leucine extracted from dried blood spots used in neonatal screening is described. The amino acids are eluted from a 3-mm filter paper
A general procedure is described for developing HPLC-based assays for nucleotidases by using a 20-mm C-18 reversed-phase column. The method is applied on the determination of pyrimidine 5'-nucleotidase activity in human erythrocytes lysate and plasma. The activity is detected using isocratic conditi