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IS421, a new insertion sequence in Escherichia coli

✍ Scribed by Showbu Sato; Yukari Nakada; Akiko Shiratsuchi


Book ID
115921451
Publisher
Elsevier Science
Year
1989
Tongue
English
Weight
655 KB
Volume
249
Category
Article
ISSN
0014-5793

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IS30, a new insertion sequence of Escher
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Three independent spontaneous mutations of prophage P1 affecting the ability of the phage to reproduce vegetatively are due to the insertion of a mobile genetic element, called IS30. The same sequence is also carried in the R plasmid NR 1-Basel, but not in the parental plasmid NR 1. Southern hybridi

Characterization of the new insertion se
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IS91 is a 1.85 kb insertion sequence originally resident in the ~-hemolytic plasmid pSU233. The element was transposed sequentially from this plasmid to pA-CYC184, to R388, and to pBR322. Both cointegrates and simple insertions of the element were obtained. A detailed restriction enzyme map of the e