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IS4 is still found at its chromosomal site after transposition to galT

✍ Scribed by Klaer, Roland ;Pfeifer, Dietrich ;Starlinger, Peter

Publisher: Springer
Year: 1980
Tongue: English
Weight: 341 KB
Volume: 178
Category: Article
ISSN: 0026-8925
DOI: 10.1007/bf00270473

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✦ Synopsis

IS4-DNA has been hybridized to separated DNA fragments of E. coli K12 strain M28 and to three mutants caused by transposition of IS4 to galT. The parental strain shows one band hybridizing to IS4 representing one copy of IS4 in the chromosome. The mutants have this copy retained and show in addition a second band corresponding to the IS4 copy in galT. The experiments support the hypothesis that transposition of IS4 is accompanied by replication of the element.

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IS4 at its chromosomal site in E. coli K

IS4 at its chromosomal site in E. coli K12

✍ Klaer, Roland ;Starlinger, Peter 📂 Article 📅 1980 🏛 Springer 🌐 English ⚖ 666 KB

IS4 DNA has been isolated in pure form. Hybridization of this DNA against restricted DNA of several E. coli K12 strains by Southern's blotting technique has shown that, in most strains, only one copy of IS4 is present. Though the restriction fragments around this site differ in size, IS4 can be show