Is the white-ivory assay of Drosophila melanogaster a useful tool in genetic toxicology?
✍ Scribed by José Antonio Ferreiro; Sofía Consuegra; Luisa María Sierra; Miguel Angel Comendador
- Publisher
- John Wiley and Sons
- Year
- 1997
- Tongue
- English
- Weight
- 98 KB
- Volume
- 29
- Category
- Article
- ISSN
- 0893-6692
No coin nor oath required. For personal study only.
✦ Synopsis
The white-ivory assay of Drosophila is based on the the three antimetabolites/nucleotide pool inhibitors detection of reversions to wild-type phenotype of tested were negative. The other chemical groups ommatidia with the white-ivory mutation. A tandem showed disparate results, since some chemicals quadruplication of this gene is used in order to in-were positive, whereas others were negative in crease the reversion probability. Although the exact each group. mechanism implicated in reversion is not known, A comparison with the results obtained in the w/ revertant spots are believed to arise as a conse-w / and mwh/flr 3 assays shows that the w i assay quence of intrachromosomal recombination or re-detects a more restricted spectrum of damages than lated phenomena.
those, although, with respect to carcinogenicity, its Since the white-ivory assay has not been broadly sensitivity (0.76, with the 62 chemicals tested until used, the number of chemicals tested until now is still now) is similar to that estimated for the mentioned limited. In this work, we have assayed 25 chemicals somatic assays. belonging to several chemical groups, i.e., cross-
The conclusion of this work, then, is that the w i linking agents, DNA-topoisomerase inhibitors, anti-assay is not recommended as a general screening metabolites/nucleotide pool inhibitors, cyclic-ad-test, because the background reversion frequenduct inducers, halogenated hydrocarbons, bulky-cies show a high variability among solvents, the adduct inducers, intercalating agents, oxidative range of lesion-recognition is lower than in the w/ damage inducers, and a multiple damage inducer, w / and mwh / flr 3 SMARTs, and the mechanism to validate this test. Cross-linking agents, haloge-implicated in the white-ivory reversion is poorly nated hydrocarbons, and the multiple damage in-understood.