Is radiation damage dependent on the dose rate used during macromolecular crystallography data collection?

✍ Scribed by Leiros, Hanna-Kirsti S. ;Timmins, Joanna ;Ravelli, Raimond B. G. ;McSweeney, Seán M.

• Book ID: 104478164
• Publisher: International Union of Crystallography
• Year: 2006
• Tongue: English
• Weight: 697 KB
• Volume: 62
• Category: Article
• ISSN: 0907-4449
• DOI: 10.1107/s0907444905033627

✦ Synopsis

This paper focuses on the radiation-damage effects when applying the same total X-ray dose to protein crystals at different dose rates. These experiments have been performed on both a selenomethionated protein and on bovine trypsin using dose rates that span nearly two orders of magnitude. The results show no clear dose-rate effect on the global indicators of radiation damage, but a small measurable dose-rate effect could be found when studying specific radiation damage. It is hypothesized that this observed dose-rate effect relates to differences in the steady-state free-radical concentration.