Iron uptake by hepatopancreas brush border membrane vesicles (BBMV) of the lobster (Homarus americanus)

✍ Scribed by Aslamkhan, Amy G. ;Ahearn, Gregory A.

• Book ID: 102336692
• Publisher: John Wiley and Sons
• Year: 2003
• Tongue: English
• Weight: 101 KB
• Volume: 295A
• Category: Article
• ISSN: 0022-104X
• DOI: 10.1002/jez.a.10223

✦ Synopsis

Abstract

The uptake of ^55^Fe^2+^ and solubilized ^55^Fe^3+^ into brush border membrane vesicles prepared from the hepatopancreas of the Atlantic lobster (Homarus americanus) was investigated. Non‐specific surface binding of ^55^Fe^2+^ at equilibrium to the vesicular surface approximated 57% of total ^55^Fe^2+^ uptake. ^55^Fe^2+^ uptake showed temperature sensitivity and was trans‐stimulated by a Ca^2+^ gradient (at 5mM) directed out. Equilibrated ^59^Fe^2+^ exchanged for both Cd^2+^ and cold Fe^2+^. The data obtained in this study are suggestive that at least a portion of ferrous iron absorption may occur by a divalent exchanger mechanism. J. Exp. Zool. 295A:145–150, 2003. © 2003 Wiley‐Liss, Inc.