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Iridoid and Aromatic Glycosides from Scrophularia ningpoensisHemsl. and Their Inhibition of [Ca2+]i Increase Induced by KCl

✍ Scribed by Bin Chen; Yan Liu; Hong-Wei Liu; Nai-Li Wang; Bao-Feng Yang; Xin-Sheng Yao


Publisher
John Wiley and Sons
Year
2008
Tongue
English
Weight
287 KB
Volume
5
Category
Article
ISSN
1612-1872

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✦ Synopsis


Abstract

Bioassay‐guided fractionation of EtOH extract of the roots of Scrophularia ningpoensis Hemsl. resulted in the isolation of three new iridoid glycosides, i.e., 6″‐O‐caffeoylharpagide (1), 6″‐O‐feruloylharpagide (2), and 6″‐Oβ‐glucopyranosylharpagoside (3), and five new aromatic glycosides, i.e., 2‐(3‐hydroxy‐4‐methoxyphenyl)ethyl Oα‐arabinopyranosyl‐(1→6)‐Oα‐rhamnopyranosyl‐(1→3)‐Oβ‐glucopyranoside (4), phenyl Oβ‐xylopyranosyl‐(1→6)‐Oβ‐glucopyranoside (5), 3‐methylphenyl Oβ‐xylopyranosyl‐(1→6)‐Oβ‐glucopyranoside (6), 6‐O‐cinnamoyl β‐fructofuranosyl‐(2→1)‐Oα‐glucopyranosyl‐(6→1)‐Oα‐glucopyranoside (7), and 6‐O‐feruloyl β‐fructofuranosyl‐(2→1)‐Oα‐glucopyranosyl‐(6→1)‐Oα‐glucopyranoside (8), together with four known compounds, i.e., 6″‐Oα‐D‐galactopyranosyl harpagoside (9), 6″‐O‐(p‐coumaroyl) harpagide (10), harpagoside (11), and angoroside C (12). Activity of the isolated compounds on [Ca^2+^]~i~ increase induced by KCl was evaluated on rat cardiac myocytes using confocal laser scanning microscopy. Iridoid glycosides 1, 10, and 11, and aromatic glycosides 5 and 6 significantly inhibited the increase of [Ca^2+^]~i~ induced by KCl at 100 μM.