Ionic requirement for epinephrine stimulation of frog skin gland secretion

✍ Scribed by Seldin, Jonathan P. ;Hoshiko, T.

• Book ID: 102334761
• Publisher: John Wiley and Sons
• Year: 1966
• Tongue: English
• Weight: 376 KB
• Volume: 163
• Category: Article
• ISSN: 0022-104X
• DOI: 10.1002/jez.1401630112

✦ Synopsis

The outer surface of isolated frog (R. pipiens) skin was observed through a dissection microscope during addition of epinephrine (2 x 10-8 w/v) to the inside bathing solution. Small droplets formed at pore openings within 30 seconds; the process appeared to be complete within 1 to 2 minutes. After washing out the bathing solution and a 30 minute recovery period, the response could be repeated up to six times. The gland requires NaCl for multiple responses. Neither added Ca nor added K is required. Cs and K may replace Na but only to a minor extent. Mg, Rb and Li could not substitute for Na. Chloride i s the preferred anion, and while NOs may be a partially effective substitute, I and SO4 were not.