𝔖 Bobbio Scriptorium
✦   LIBER   ✦

Ion trap mass spectrometry as detector for capillary electrochromatography of peptides: Possibilities and limitations

✍ Scribed by Marco Gaspari; Marjan Guček; Karin Walhagen; Rob J. Vreeken; Elwin R. Verheij; Ubbo R. Tjaden; Jan Van der Greef

Publisher
John Wiley and Sons
Year
2001
Tongue
English
Weight
114 KB
Volume
13
Category
Article
ISSN
1040-7685

No coin nor oath required. For personal study only.

✦ Synopsis

Abstract

Capillary electrochromatography has been coupled to an ion trap mass spectrometer via a sheath flow electrospray interface. Peptide analysis was performed on 25 cm columns packed with C~18~‐bonded silica particles, and very fast separations were obtained. Full mass spectrometry and tandem mass spectrometry (MS/MS) detection were evaluated in terms of sensitivity and scanning speed. The separation of seven selected peptides and their detection by MS/MS in selected reaction monitoring mode was demonstrated. The performance of this sheath flow interface also was compared with the previously reported sheathless interface. Despite the loss in sensitivity (20–40‐fold), the sheath flow interface was shown to be superior in terms of ruggedness, and allowed the use of higher electric fields to achieve faster analysis times. © 2001 John Wiley & Sons, Inc. J Micro Sep 13: 243–249, 2001

📜 SIMILAR VOLUMES

Optimization of a quadrupole ion storage
Optimization of a quadrupole ion storage trap as a source for time-of-flight mass spectrometry
✍ Beni B. Dangi; Kent M. Ervin 📂 Article 📅 2012 🏛 John Wiley and Sons 🌐 English ⚖ 386 KB

Designs of a quadrupole ion trap (QIT) as a source for time‐of‐flight (TOF) mass spectrometry are evaluated for mass resolution, ion trapping, and laser activation of trapped ions. Comparisons are made with the standard hyperbolic electrode ion trap geometry for TOF mass analysis in both linear and

A new strategy utilizing electrospray io
A new strategy utilizing electrospray ionization-quadrupole ion trap mass spectrometry for the qualitative determination of GnRH peptides
✍ Tanya R. Myers; Gabor Patonay 📂 Article 📅 2006 🏛 John Wiley and Sons 🌐 English ⚖ 475 KB

## Abstract Numerous forms of the neurotransmitter GnRH have been discovered in vertebrates and invertebrates. Methods used for identification of these peptides are laborious and often require the application of multiple, confirmatory techniques. In this study, we investigate whether HPLC‐MS/MS and

Development of a low-flow multiplexed in
Development of a low-flow multiplexed interface for capillary electrophoresis/electrospray ion trap mass spectrometry using sequential spray
✍ Chao-Jung Chen; Fu-An Li; Guor-Rong Her 📂 Article 📅 2008 🏛 John Wiley and Sons 🌐 English ⚖ 383 KB 👁 1 views

## Abstract A multiplexed CE‐MS interface using four low‐flow sheath liquid ESI sprayers has been developed. Because of the limited space between the low‐flow sprayers and the entrance aperture of the ESI source, multichannel analysis is difficult using conventional rotating plate approaches. Inste