Ion transport by ouabain resistant and sensitive ehrlich ascites carcinoma cells

✍ Scribed by E. Mayhew

• Publisher: John Wiley and Sons
• Year: 1972
• Tongue: English
• Weight: 730 KB
• Volume: 79
• Category: Article
• ISSN: 0021-9541
• DOI: 10.1002/jcp.1040790314

✦ Synopsis

Cell division, net Na+-K+ and amino-acid transport of cultured Ehrlich ascites is reversibly inhibited by Ouabain at a final concentration of 1 X lO-3M. A line of Ehrlich ascites cells resistant to the growth inhibiting effects of Ouabain has been developed. These cells behave similarly to Ouabainsensitive cells in the following respects doubling time, S phase time, chromosome number, cell surface charge density, rate of incorporation of CI4 Uridine and "-Thymidine, sensitivity to Digoxin and Digitoxin, steady state Na+, K+ levels and rate of loss of K+ and gain of Na+ in cold.

Ouabain resistant cells differ from sensitive cells only with respect to the effect of ouabain on active Na+, K+ transport. Although Ouabain inhibits active Na+, K+ transport in sensitive cells it has no significant effect in resistant cells.