Ion-Selective Microelectrodes and Their Use in Excitable Tissues

✍ Scribed by Jiří Koryta (auth.), Eva Syková, Pavel Hník, Ladislav Vyklický (eds.)

• Publisher: Springer US
• Year: 1981
• Tongue: English
• Leaves: 348
• Edition: 1
• Category: Library

✦ Synopsis

The Symposium on "Ion Selective Microelectrodes and Their Use in Excitable Tissues" was held in Prague from July 8-11, 1980. It was organized by the Institute of Physiology of the Czechoslovak Academy of Sciences as a satellite symposium of the XXVIII Interna­ tional Congress of Physiological Sciences in Budapest. Sixty parti­ cipants met for three days in the historical setting of the Strahov Monastery. The informal and relaxed atmosphere of the scientific sessions, together with the social programme made the meeting a success and helped to cement old friendships and to form new ones. The organizers were happy to welcome as participants representatives from most of the laboratories working with ion-selective microelectrodes (ISMs) in the world. Neurophysiological research with liquid ion-exchanger ISMs in the Prague laboratory was started as early as 1971 due to the fact that one of us (P. H. ) had the opportunity of learning the technique directly from Dr. J. L. Walker in Salt Lake City. It was thanks to his patience and, later, his courtesy in providing us with the liquid ion-exchanger for potassium and silicone that we were able to get a start in what turned out to be, in a year or so, very strong international competition. This volume contains the papers presented at the Symposium. It is divided according to the actual sessions with names of the chair­ men, who helped the organizers to make the Symposium a real success.

✦ Table of Contents

Front Matter....Pages i-xi
Front Matter....Pages 1-1
Theory of Ion-Selective Electrodes....Pages 3-11
New Ion Selective Liquid Membrane Microelectrodes....Pages 13-23
Sensitivity of K + -Selective Microelectrodes to pH and Some Biologically Active Substances....Pages 25-39
Double-Barrel Ion Selective [K + , Ca 2+ , Cl − ] Coaxial Microelectrodes (ISCM) for Measurements of Small and Rapid Changes in Ion Activities....Pages 41-45
Determination of Selectivity Coefficients of Ion-Selective Microelectrodes....Pages 47-52
Front Matter....Pages 53-53
Intracellular Potassium and Chloride Measurements in Sheep Cardiac Purkinje Fibers....Pages 55-66
Intracellular Electrochemical Potentials: Skeletal Muscle vs . Epithelial Steady-State vs . Kinetics....Pages 67-89
Measurement of Intracellular Calcium Activities....Pages 91-94
pH Related Potassium Movements in Rabbit Atrium....Pages 95-97
Intracellular Free Calcium Affects Electric Membrane Properties. A Study with Calcium-Selective Microelectrodes and with Arsenazo III in Helix Neurons....Pages 99-117
Measurement of Free Ca 2+ in Nerve Cell Bodies....Pages 119-124
Intracellular Recording of Potassium in Neurons of the Motor Cortex of Awake Cats Following Extracellular Applications of Acetylcholine....Pages 125-131
Potassium Fluxes of Isolated Nerve Endings Monitored by a Valinomycin-Activated Ion-Selective Membrane Electrode in vitro ....Pages 133-136
Front Matter....Pages 137-137
Extracellular K + Accumulation in the Spinal Cord....Pages 139-158
Extracellular Potassium and Calcium Activities in the Mammalian Spinal Cord, and the Effect of Changing Ion Levels on Mammalian Neural Tissues....Pages 159-180
Relations between Extracellular K + and Ca ++ Activities and Local Field Potentials in the Spinal Cord of the Rat During Focal and Generalized Seizure Discharges....Pages 181-185
Ion Fluxes Across the Membrane of Motoneurons During the Action of Glutamate....Pages 187-190
The Ionic Basis of the IPSP in Spinal Motoneurons of the Frog....Pages 191-194
Effects of Increased Extracellular Potassium Activity in the Frog Spinal Cord on the Flexor Reflex....Pages 195-199
Extracellular K + Activity during Electrical Stimulation of Rat Sympathetic Ganglia, Vagus and Optic Nerves in Vitro....Pages 201-204
Front Matter....Pages 137-137
Extracellular Potassium Activity and Axon Excitability....Pages 205-208
Front Matter....Pages 209-209
Extracellular Potassium, Calcium and Volume Profiles During Spreading Depression....Pages 211-223
Cortical pH and pCa in Relation to DC Potential Shifts During Spreading Depression and Asphyxiation....Pages 225-229
Changes of Extracellular Potassium in Rat Cerebellar Cortex Indicate a Reduced Na-K-Pump Activity during Acute and Chronic Li-Application....Pages 231-234
Changes in Extracellular Free Ca 2+ in the Sensorimotor Cortex of Cats During Electrical Stimulation and Iontophoretic Application of Amino-Acids....Pages 235-239
Measurements of Ion Activity in the CNS: Extracellular K + and Ca 2+ in the Hippocampus....Pages 241-245
Evoked Ionic Alterations in Brain Slices....Pages 247-259
Serotonin and GABA-Induced Fluctuations in Extracellular Ion Concentration in the Hippocampal Slice....Pages 261-265
The Slow Component of the Direct Cortical Response and Extracellular Potassium....Pages 267-272
Potassium Changes Elicited in the Feline Somatosensory Cortex by a Single Stimulus to the Peripheral Nerve....Pages 273-278
The Mechanism and Integrative Role of Extracellular Potassium Clearance in the Rat Brain....Pages 279-282
Front Matter....Pages 283-283
K + Accumulation and Its Physiological Regulatory Role in Heart Muscle....Pages 285-298
Measurement of Intracellular Ionic Calcium Concentration in Guinea Pig Papillary Muscle....Pages 299-301
Intracellular Na Activity During K or Rb Activated Response in Sheep Purkinje Fibres—Correlation with Changes in Membrane Potential....Pages 303-306
Interaction between pH i and pCa i in Cardiac Tissue....Pages 307-309
The Use of ISMs in Working Skeletal Muscles and Venous Effluent Blood....Pages 311-315
Characteristics of Ca 2+ -Selective Microelectrodes and Their Application to Cardiac Muscle Cells....Pages 317-321
Front Matter....Pages 323-323
Ion-Selective Properties of the Nerve Cell Membrane....Pages 325-330
Glial Cells as Potassium Detectors....Pages 331-337
The Role of Cells in the Dispersal of Brain Extracellular Potassium....Pages 339-343
Front Matter....Pages 323-323
Movement of Potassium into Glial Cells in the Retina of the Drone, Apis mellifera, During Photostimulation....Pages 345-349
Intracellular Calcium and the Regulation of Neuronal Membrane Permeability....Pages 351-354
CNS and Na-Pump in Situ Muscle....Pages 355-355
Back Matter....Pages 357-369

✦ Subjects

Neurosciences