Ion-selective membrane electrodes for the determination of pyrantel with low protein interference

✍ Scribed by Ralph Aubeck; Norbert Hampp

• Publisher: Elsevier Science
• Year: 1992
• Tongue: English
• Weight: 457 KB
• Volume: 256
• Category: Article
• ISSN: 0003-2670
• DOI: 10.1016/0003-2670(92)85352-7

✦ Synopsis

A comparison was made of Ion-selectwe poly(vmyl chloride) hqmd membrane electrodes for the determmatlon of pyrantel (PY) based on four different Ion-pamng agents, VU, tetraphenylborate (TPB), dlplcrylammate (DIPIC), remeckate (REINE) and tungstosdlcate @IWO) The four electrodes showed slmdar detectlon hmlts of l-2 pg ml-' and nearly the same hnear ranges of 1 X lo-'-> lo-* mol I-' for pyrantel m 100 mM sodmm phosphate-buffered solutions of pH 7 0 Slgmfxant differences between the electrodes were observed m protem-contammg solutions The detectlon hmlts of the electrodes with the aon pairs PY-REINE and PY-SIWO were not affected by a background of 6 7 g 1-l (10m4 mol 1-l) bovme serum albumm (BSA), but the PY-TPB-and PY-DIPIC-based electrodes were 6-8 times less sensltlve m protem-contammg solutions Of the two protem-msensltlve electrodes PY-REINE and PY-SIWO, the latter IS advantageous for the determmatlon of PY m bIologIca media as It 1s about hvlce as sensltwe as the PY-REINE electrode Inorgamc cattons did not Influence the electrode response even when present m high concentrations, but some hpophthc alkaloids such as berberme and papaverme did