Ion-Pair High-Performance Liquid Chromatographic Retention Behavior of Copper(II)–1-Oxa-4,7,10,13- tetraazacyclopentadecane Complex
✍ Scribed by Yongsoon Chung; Bongjoon Kim; Jongyeon Hwang; Chang-Suk Kim
- Publisher
- Elsevier Science
- Year
- 1999
- Tongue
- English
- Weight
- 54 KB
- Volume
- 62
- Category
- Article
- ISSN
- 0026-265X
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✦ Synopsis
The ion-pair high-performance liquid chromatographic retention behavior of copper(II)-1-oxa-4,7,10,13-tetraazacyclopentadecane (Cu(II)-OTACP) complex is discussed with data from indirect spectrophotometric detection on the Bondapak CN column. The mobile phase was acetonitrile solution (MeCN:H 2 O 20:80) containing sodium dodecyl sulfate (SDS) as ion-pairing reagent and sodium 1-naphthalenesulfonate (SNS) as detection reagent. The effects of the concentration of SDS and OTACP added to the sample solution to form the Cu(II)-OTACP complex on the capacity factor of the complex, kЈ, are illustrated. As a consequence of the study, it was found that two peaks anticipated for the 1:1 and 1:2 mole ratio complexes appeared, and the peak anticipated for the 1:2 mole ratio complex could be used to determine the Cu(II) ion. The method has been applied in the determination of Cu(II) ion in waste water and serum.