Ion-pair extraction of bile acids with Lipidex gel

✍ Scribed by Arne Dyfverman; Jan Sjövall

• Publisher: Elsevier Science
• Year: 1983
• Tongue: English
• Weight: 549 KB
• Volume: 134
• Category: Article
• ISSN: 0003-2697
• DOI: 10.1016/0003-2697(83)90301-9

✦ Synopsis

A new method for the extraction of bile acids from aqueous solutions, urine, plasma, and bile is described. A buffered solution of decyltrimethylammonium bromide is added to the sample to give a 0.03 M concentration of the counter-ion. The mixture is passed through a bed of Lipidex 1000, which is then washed with the buffered solution of counter-ion followed by water. The decyltrimethylammonium salts of bile acids are sorbed by the Lipidex and are eluted with methanol. Recoveries of unconjugated, taurine- and glycine-conjugated, sulfated, and glucuronidated bile acids are close to 100%. Unconjugated bile acids can also be quantitatively extracted from aqueous solutions and urine after acidification with acetic acid.