Ion-exchange separation and quantitative determination of dimethyl sulfoxide

✍ Scribed by L.H. Krull; Mendel Friedman

• Publisher: Elsevier Science
• Year: 1967
• Tongue: English
• Weight: 304 KB
• Volume: 26
• Category: Article
• ISSN: 1873-3778
• DOI: 10.1016/s0021-9673(01)98888-0

✦ Synopsis

with dyes numbered r, 3, 15, 31 or 32 was performed. The stained BSA was dialyzed against Miller-Golder buffer (pH 7.3, p o.1)2 for 48 h before being added to the resin beads. Though definite color still persisted in the BSA in all cases, no penetration of colored material into the beads was observed.

It is apparent that resin beads of the polystyrene type are more permeable to molecules up to molecular weights of 1628 than'?&ight have been supposed. This suggests that separations of substances in this size range on such resins depend very little on simple permeability phenomena, and that the rationale behind choosing a resin of a given cross-linkage for a given purpose needs reconsideration.

The simple staining methods described permit the detection of accidental contamination of a cation exchanger with an anion exchanger, or vice vema. The experiment also indicates that particles as large as BSA cannot penetrate these beads. The uniformity of the dyed resin beads suggests that they may be useful as standards for the calibration of spectrophotometric systems.