A rapid procedure for the large-scale purification of pig heart nucleoside diphosphate kinase is described. The purification procedure involves extraction of the enzyme, absorption on Cibacron Blue 3G-A Sepharose, elution with ATP, ammonium sulfate precipitation, heat treatment, and rechromatography
Ion-exchange properties of cibacron blue 3G-A sepharose (blue sepharose) and the interaction of proteins with cibacron blue 3g-a
โ Scribed by Ioan Lascu; Horea Porumb; Tudor Porumb; Ileana Abrudan; Cornelia Tarmure; Ioan Petrescu; Elena Presecan; Ioan Proinov; Marius Telia
- Publisher
- Elsevier Science
- Year
- 1984
- Tongue
- English
- Weight
- 700 KB
- Volume
- 283
- Category
- Article
- ISSN
- 1873-3778
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