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Ion-exchange high-performance liquid chromatography analysis of oligodeoxyribonucleotide phosphorothioates

โœ Scribed by Valeri Metelev; Sudhir Agrawal

Publisher
Elsevier Science
Year
1992
Tongue
English
Weight
545 KB
Volume
200
Category
Article
ISSN
0003-2697

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โœฆ Synopsis

Oligodeoxynucleotide-containing phosphorothioate backbones have been used to regulate viral as well as cellular gene expression. The studies carried out in tissue culture have shown promising results on the use of oligonucleotide phosphorothioates as antiviral agents and, at present, study is underway to develop these oligonucleotide analogues as chemotherapeutic agents. To analyze and purify oligonucleotide analogues, high-performance liquid chromatography using weak anion exchange column has been described. The separation of oligonucleotide phosphorothioate is found to be length dependent.

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