Ion-Exchange Chromatography of Proteins

✍ Scribed by Shuichi Yamamoto (Author); Kazahiro Nakanishi (Author); Ryuichi Matsuno (Author)

Publisher: CRC Press
Year: 1988
Leaves: 417
Edition: 1
Category: Library

No coin nor oath required. For personal study only.

✦ Synopsis

Showing chemists how to predict ion-exchange chromatography (IEC) separation behaviour and how to determime appropriate operating conditions, this reference illustrates procedures, apparatus and types of ion exchanges, emphasizing the design and application of large-scale IEC. Complete with more than 100 useful tables and diagrams, this text explains the effects of each variable on separation behaviour; compares models and equations describing behaviour of proteins in IEC with experimental results; introduces design calculation procedures for scale up, adapting IEC for individual requirements; discusses parameters affecting separation behaviour, including adsorption equilibria, stationary phase diffusion and axial dispersion; provides examples of applications, such as high or medium performance IEC and large scale operations and reviews both theorectical and experimental literature. This text serves as an reference for analytical, agricultural, food, and pharmaceutical chemists, chromatographers, food scientists and technologists, biochemists and biotechnologists.

✦ Table of Contents

1 INTRODUCTION, 2 THEORETICAL ASPECTS, 3 ION-EXCHANGE EQUILIBRIA, 4 DIFFUSION IN THE ION EXCHANGER, 5 AXIAL DISPERSION IN THE ION-EXCHANGE COLUMN, 6 EXPERIMENTAL METHODS AND APPARATUS, 7 ION EXCHANGERS, 8 FACTORS AFFECTING SEPARATION BEHAVIOR, 9 LARGE-SCALE OPERATION 9. 10 DESIGN CALCULATION PROCEDURE, APPENDIX.

✦ Subjects

Physical Sciences;Chemistry;Analytical Chemistry;Chromatography

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