Ion cluster techniques in drug metabolism: An example of the use of mixtures of the [12C]:[14C] isotopic forms of the synthetic prostaglandin cloprostenol (‘Estrumate’) to Facilitate Metabolite Identification

✍ Scribed by G. R. Bourne; S. R. Moss; P. J. Phillips; J. T. A. Webster; D. F. White

• Publisher: John Wiley and Sons
• Year: 1979
• Tongue: English
• Weight: 165 KB
• Volume: 6
• Category: Article
• ISSN: 1076-5174
• DOI: 10.1002/bms.1200060811

✦ Synopsis

The synthetic prostaglandin analogue cloprostenol has been prepared radiolabelled with 14C. The isotopic abundance of 14C at position C-15 was greater than 90% of the theoretical maximum. We have utilizted the high abundance of the 14C isotope for metabolism studies by preparing mixtures of [12C]:[14C]cloprostenol such that fragments detected by mass spectrometry contained characteristic isotope clusters analogous to those often obtained using stable isotopes.