Iodination of proteins, glycoproteins, and peptides using a solid-phase oxidizing agent, 1,3,4,6-tetrachloro-3α,6α-diphenyl glycoluril (Iodogen)

✍ Scribed by Philip R.P. Salacinski; Charles McLean; Jane E.C. Sykes; Vicky V. Clement-Jones; Philip J. Lowry

• Book ID: 102983516
• Publisher: Elsevier Science
• Year: 1981
• Tongue: English
• Weight: 901 KB
• Volume: 117
• Category: Article
• ISSN: 0003-2697
• DOI: 10.1016/0003-2697(81)90703-x

✦ Synopsis

A new, commercially available oxidizing agent, 1,3,4,6-tetrachloro-3a,6a-diphenyl glycoluril (Iodogen) was compared with chloramine-T and solid-phase Iactoperoxidase in the radioiodination of proteins, glycoproteins, and peptides. A method for performing low-level iodinations is described and was used to determine maximum '*'I incorporation. Iodinated proteins were purified on analytical gel filtration columns and peptides by reverse-phase high-performance liquid chromatography. Both methods were designed to analyze the tracers for the presence of aggregate and breakdown products caused by the iodination. All tracers prepared were tested in antibody dilution and dose-response curves in their respective radioimmunoassays. Results indicate that Iodogen can be used for a wide range of proteins and peptides, can permit theoretical iodine incorporation with minimal oxidation damage, and can produce tracer stable for up to 3 months.