Involvement of p21ras activation in T cell CD69 expression

✍ Scribed by Daniele d'Ambrosio; Doreen A. Cantrell; Luigi Frati; Angela Santoni; Roberto Testi

• Publisher: John Wiley and Sons
• Year: 1994
• Tongue: English
• Weight: 577 KB
• Volume: 24
• Category: Article
• ISSN: 0014-2980
• DOI: 10.1002/eji.1830240319

✦ Synopsis

Involvement of p2lraS activation in T cell CD69 expression

The involvement of p21ras in the induction of the early activation antigen CD69 was investigated in T cells. Expression of a v-Ha-rus coding for a constitutively active ras protein in Jurkat cells resulted in CD69 induction on the cell surface. Transfected ras was shown to be constitutively activated and functionally efficient, since it could be immunoprecipitated in the guanosine triphosphate (GTP)-bound form and it induced transactivation of an AP-1 consensuschloramphenicol acetyltransferase reporter gene. The requirement for ras activation in T cell receptor (TcR) CD3-mediated CD69 induction was also investigated. The expression of a dominant negative c-Ha-rus-N17 mutant markedly reduced the amount of GTP that could be immunoprecipitated from ras proteins after TcR/CD3 triggering in Jurkat cells, and concomitantly decreased TcRICD3-mediated CD69 induction. These results suggest a central role for ras in TcRKD3-mediated CD69 expression in T cells.