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Involvement of matrix metalloproteinase-2 activity in invasion and metastasis of pancreatic carcinoma

✍ Scribed by Takatomo Koshiba; Ryo Hosotani; Michihiko Wada; Yoshiharu Miyamoto; Koji Fujimoto; Jeon-Uk Lee; Ryuichiro Doi; Shigeki Arii; Masayuki Imamura

Publisher: John Wiley and Sons
Year: 1998
Tongue: English
Weight: 185 KB
Volume: 82
Category: Article
ISSN: 0008-543X
DOI: 10.1002/(sici)1097-0142(19980215)82:4<642::aid-cncr5>3.0.co;2-n

No coin nor oath required. For personal study only.

✦ Synopsis

Background:

Activation of matrix metalloproteinase-2 (mmp-2) has been implicated in the progression, invasion, and metastasis of various cancers, but little information is available with regard to its role in pancreatic carcinoma with poor prognosis.

Methods:

Gelatin zymography was used for the detection of latent and activated forms of mmp-2 and mmp-9 in 13 normal pancreatic tissue specimens, 14 chronic pancreatitis tissue specimens, and 33 pancreatic carcinoma tissue specimens. the gelatinase activity was quantified by densitometer, and the 66-kilodalton (kda)/(66-kda + 72-kda) ratio was calculated as the mmp-2 activation ratio. western blot analysis was performed to confirm the zymographic profile.

Results:

Latent forms of mmp-2 and mmp-9 were detected in all samples of pancreatic carcinoma, chronic pancreatitis, and normal pancreatic tissue. the expression rate of the mmp-2 activated form in pancreatic carcinoma tissue specimens was 100% (33 of 33) but that of mmp-9 was 21%. the mmp-2 activation ratio in pancreatic carcinoma tissue specimens was significantly higher than that of chronic pancreatitis and normal pancreatic tissue specimens. the mmp-2 activation ratio in pt3 tumors was significantly higher than that in pt1 tumors. the mmp-2 activation ratio also was significantly higher in pancreatic carcinoma specimens with histologically positive regional lymph node metastasis and distant metastasis than those without metastasis. the mmp-2 activation ratio observed in patients who developed postresection recurrence within 6 months was significantly higher than that in patients without recurrence at 6 months.

Conclusions:

The results of the current study indicate that mmp-2 activation plays a significant role in tumor invasion and metastasis in pancreatic carcinoma.

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