Involvement of Intracellular Ca2+in the Regulation of Bovine Leukemia Virus Expression

✍ Scribed by Bondzio, Angelika; Abraham-Podgornik, Anita; Blankenstein, Petra; Risse, Siegfried

• Book ID: 121442131
• Publisher: Walter de Gruyter GmbH & Co. KG
• Year: 2001
• Tongue: English
• Weight: 339 KB
• Volume: 382
• Category: Article
• ISSN: 1431-6730
• DOI: 10.1515/BC.2001.050

✦ Synopsis

The calcium ionophore A23187, which was used to increase the intracellular calcium concentration ([Ca2+]i), was analyzed for effects on bovine leukemia virus (BLV) expression in two BLV infected cell lines. To clarify the role of intracellular free calcium in this response, [Ca2+]i was measured during ionophore treatment with the fluorescent calcium indicator Fura-2. Elevation of intracellular calcium under these conditions caused an enhancement of BLV gp51 and p24 synthesis as well as an activation of the BLV long terminal repeat (LTR) in a dose-dependent manner. Furthermore, it was observed that elevated levels of intracellular calcium following A23187 stimulation lead to activation of NF-kappaB. Based on inhibitor studies, we hypothesize that the effect of A23187 on BLV expression appears to be mediated by PKC.