Involvement of DNA gyrase in the replication and transcription of mycobacteriophage 13 DNA
โ Scribed by V. Nagaraja; K.P. Gopinathan
- Book ID
- 115912101
- Publisher
- Elsevier Science
- Year
- 1981
- Tongue
- English
- Weight
- 487 KB
- Volume
- 127
- Category
- Article
- ISSN
- 0014-5793
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A temperature-sensitive mutant of E. coli with a defective DNA gyrase B subunit has been obtained. The mutation is expressed in the thermolability of DNA gyrase in vitro and in DNA relaxation in vivo. DNA replication in the mutant does not stop under non-permissive conditions; its rate gradually fal
The effects of oxolinic acid and novobiocin, two known inhibitors of DNA gyrase, on in vivo transcription in E. coli were investigated. The drugs inhibit the incorporation of 3H-uridine into RNA. It is shown that the effect is due to a direct influence of DNA gyrase on transcription, independent of